Trascriptome profiling of murine Embryonic Stem cells under different culture conditions
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https://www.ncbi.nlm.nih.gov/sra/SRP134935
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Mouse Embryonic Stem (ES) cells have been cultured under chemically defined conditions in the absence of feeder cells on Gelatin coated dishes, for 5 passages under 4 different combinations of LIF and GSK3 or MEK inhibitors. Their trascriptome have been analysed by RNA sequencing. The ES cell line used, RexGFPd2, contains a destablised GFP protein in the Zfp42/Rex1 locus and was derived under 2i conditions, as described in Kalkan et al. 2017. Overall design: Examination of transcriptome by RNA sequencing of mES cells under 4 different culture conditions
创建时间:
2021-03-03



