Hordeum vulgare subsp. vulgare cultivar 'Morex' assembly (CSS reads with Falcon)

Sequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial compute resources and often several complementary sequencing and mapping approaches. The recent development of fast and accurate long-read sequence assembly by circular consensus sequencing (CCS) on the PacBio platform may greatly increase the scope of plant pan-genome projects. Here, we compare current long-read sequencing platforms regarding their ability to rapidly generate contiguous sequence assemblies in pan-genome studies. Barley CSS reads assembled with Falcon. Sequences were provided by the HudsonAlpha Genome Sequencing Center.