Coordination of the host Vps4-Vta1 complex and the viral core protein Ac93 facilitates entry of Autographa californica multiple nucleopolyhedrovirus budded virions

The endosomal sorting complex required for transport (ESCRT) is a protein machine mediating membrane scission. In intraluminal vesicles (ILVs) formation, ESCRT-0 targets cargoes and recruits ESCRT-I/-II to create membrane invagination, whereas ESCRT-III coordinates with the AAA ATPase Vps4 and its cofactor Vta1 to catalyze the membrane fission. Recently, ESCRT-I/-III and Vps4 were found to involve in entry of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, the necessity of other ESCRT components and the interplay of viral proteins and ESCRTs in regulating the virus entry remain elusive. Here, we identified ESCRT-0 (Hse1 and Vps27), ESCRT-II (Vps22, Vps25, and Vps36), and Vta1 of S. frugiperda. RNAi depletion of Vta1 but not the components of ESCRT-0 or ESCRT-II in Sf9 cells significantly reduced budded virus (BV) production. Quantitative PCR together with confocal microscopy analyses indicated that Vta1 was required for internalization and endosom..., , , # Coordination of the host Vps4-Vta1 complex and the viral core protein Ac93 facilitates entry of Autographa californica multiple nucleopolyhedrovirus budded virions

[https://doi.org/10.5061/dryad.g1jwstr2k](https://doi.org/10.5061/dryad.g1jwstr2k)

## Description of the data and file structure

The data files contain the LacZ ((β-galactosidase) and GUS (β-glucuronidase) activities and the virus titers. The extended data file contains the supplemental figures.

For measurement of LacZ and GUS activities, a brief description of the methods as below (detailed Materials and Methods see the associated paper):

Sf9 cells in 12-well plates (200000 cells/well) were transfected with the dsRNA of gfp, Vps4, or Vta1 (5 g/well for each gene) using the standard CaPO4 precipitation method. At 48 h post-transfection (p.t.), the cells were transfected again with the bacmid DNA of AcMNPV-LacZGUS (4 g/well). After transfecting the viral DNA for 24 h, the cells were lysed using 1% Triton X-100 containin...,