Differential Gene expression of furin knockout (KO) DLD1, HCA7 and HT29 colorectal cancer cells

We created furin KO cells in three colorectal cancer cell lines (DLD1, HCA7 and HT29) using CRISPR-Cas9 genome editing. And then we performed RNA-Seq analysis in furin KO colorectal cancer cells to identify potential effect of furin on gene expression patterns in DLD1, HCA7 and HT29 cells. Overall design: mRNA profiles of DLD1 cells (WT or furin KO), HCA7 cells (WT or furin KO) and HT29 cells (WT or furin KO) using RNA-Seq.