Genomic Run On (GRO): determination of the nascent transcriptional rates and mRNA levels in several yeast mutants (PUB1 and NGR1)

We analyzed the effect of PUB1 and NGR1 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a deletion mutants comparing with a wild type in both glucose (YPD) and galactose (YPGal) media本研究在葡萄糖（YPD）与半乳糖（YPGal）两种培养基中，以野生型菌株为对照，对缺失突变体开展GRO实验，以此分析PUB1与NGR1对转录速率、mRNA稳定性及mRNA水平的影响。