Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

We treated mESCs with 50mM lactate to examine its impact on mESC epigenome. Interestingly, we found that lactate supplementation stimulated H3K18 lactylation accumulation on a subset of genes, which in turn promoted transcriptional elongation. Our results indicate that lactate supplementation expands transcriptional network of mouse ESCs. Overall design: We treated mESCs with lactate, and produced libraries to identify changes of epigenome.