Non-invasive delivery of CRISPR RNPs via virus-like particles for efficient mouse model generation

CRISPR-VIM enables genome editing in mouse embryos through non-invasive delivery of CRISPR RNPs via VLPs, eliminating the need for physical manipulation and specialized expertise. It supports diverse genome edits, including knockout, base editing, and knock-in using tools such as Cas9, ABE, CBE, and AAV-mediated HDR. CRISPR RNPs reduces off-target effects and increases editing precision, facilitating the generation of GEMMs. This protocol is compatible with zygotes and IVF-derived embryos via simple co-culture, enabling high-efficiency, high-throughput editing without specialized equipment and reducing animal use.