The IC50 values of KPT185 for growth inhibition and apoptosis in a panel of human melanoma cell lines.

A panel of human melanoma cell lines (A375, Hs294t, FO-1, Wm1366, CHL-1) and three normal human melanocyte cell lines (HEM-l, HEMn-MP, HEMn-DP) were treated with a range of KPT-185 concentrations (0–10.24 µM). After 72 hr of treatment, growth inhibition was evaluated using an MTS assay, and apoptosis was evaluated via Annexin V/PI staining and flow cytometric analysis. The 5 melanoma lines and the 3 melanocyte lines had significantly different sensitivity (p = 0.0253) to KPT-185 treatment.