METHOD FOR SEPARATING AND PURIFYING RECOMBINANT HUMAN FIBRONECTIN FROM GENETICALLY ENGINEERED RICE SEED
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
Disclosed is a chromatographic method for separating and purifying a recombinant human fibronectin from a genetically engineered rice seed that expresses the fibronectin. In the method， the genetically engineered rice seed is milled， mixed with an extraction buffer， and then filtered to obtain a crude extract comprising the recombinant human fibronectin; the crude extract comprising the recombinant human fibronectin is subjected to cation exchange chromatography， so as to perform primary separation and purification， thereby obtaining a primary product comprising the recombinant human fibronectin; and the primary product is subjected to anion exchange chromatography so as to perform final separation and purification to obtain the recombinant human fibronectin as a target substance. The method is low cost and easily executed on an industrial scale. The obtained OsrhFn target substance has a SEC-HPLC purity greater than 95% with good bioactivity.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
