MG132 treatment fails to rescue the functional expression of A531V.

(A & B) Effects of the MG132 treatment (20-µM, 24 hrs) on the functional expression of WT and A531V in HEK293T cells. Left and middle panels are cell-attached patch recordings from cells with and without the MG132 treatment, respectively. The averaged current amplitudes were compared in the right panels with the number of patches shown on top of each column. The asterisk denotes a significant difference from the control (no MG132 treatment) condition (*, t-test: p(C) Effects of the MG132 treatment on the current density of A531V channels. Data were derived from whole-cell recordings. The instantaneous currents at Vm = -140 mV were used for the calculation.