High-speed atomic force microscopy images of DNA origami triangles adsorbing at mica surfaces

Each folder corresponds to a different DNA origami concentration as indicated. Imaging was performed using a JPK Nanowizard ULTRA Speed 3 (Bruker) with USC F0.3-k0.3 cantilevers (NanoWorld). The DNA origami triangles were suspended in 1x TAE (pH 8.5) containing 10 mM CaCl2 and 75 mM NaCl. For the first 10 min, images were recorded with 1 x 1 µm² scan size at a line rate of 200 Hz and a resolution of 200 x 200 pixels resulting in a frame rate of 1 fps. After about 10 min, the resolution was increased to 400 x 400 pixels, lowering the frame rate to 0.5 fps. After about another 15 min, overview images 4 x 4 µm² in size were recorded at a line rate of 20 Hz and a resolution of 2048 x 2048 pixels.