Cold atmospheric plasma improves antifungal responsiveness of Aspergillus flavus and Fusarium keratoplasticum conidia and mycelia

The purpose of this study is to evaluate sublethal cold atmospheric plasma (CAP) treatment of filamentous fungal pathogen susceptibility to commonly used antifungal drugs in vitro. Response to CAP in combination with voriconazole, fluconazole, amphotericin B, and caspofungin was evaluated in Aspergillus flavus and Fusarium keratoplasticum conidia and mycelium; conidial response to fluconazole was also assessed in three strains of F. falciforme. Conidial susceptibility to antifungal drugs alone or in combination with CAP was assessed using a modified CLSI broth microdilution assay with MIC determination and colony-forming unit (CFU) enumeration. Mycelial viability and biofilm thickness changes in response to antifungal drugs alone or in combination with CAP were assessed over 24 hours post treatment. CAP enhanced antifungal drug efficacy against all fungal species, though effects differed by drug and growth form.
CAP enhanced antifungal drug susceptibility in conidia, with the strongest ..., , # Cold atmospheric plasma improves antifungal responsiveness of Aspergillus flavus and Fusarium keratoplasticum conidia and mycelia

Dataset DOI: [10.5061/dryad.931zcrjxc](10.5061/dryad.931zcrjxc)

## Description of the data and file structure

### Files and variables

#### File: CAP_AF_Conidia_CFU_mL_Data.csv

**Description:** Colony forming unit per mL counts from fungal conidia samples treated with or without antifungal (AF) drugs (either voriconazole, fluconazole, amphotericin B, or caspofungin) at increasing doses, alone or in combination with cold atmospheric plasma (CAP) treatment. 

##### Variables

* Fungal species: Identifies fungal organism sampled
* Drug: Indicates antifungal drug selection
* Dose (ppm): Indicates concentration of antifungal drug
* CAP Condition: Indicates CAP treated versus untreated
* CFU/mL: Diagnostic outcome - counted colony forming units per milliliter 

#### File: CAP_AF_Mycelial_Metabolic_Activity_Data.csv

**Description:** Metabolic activity (measur...,