Supporting data for manuscript: A modified fully automated dried blood spot sample preparation system and its application in steroid esters detection

This project focuses on developing an efficient and sensitive method for detecting steroid esters in dried blood spots (DBS) to support anti-doping efforts. By optimizing a fully automated DBS processing system with an independent desorption pump, we established a high-throughput screening method for 30 steroid esters and a confirmatory method for 26 targets using HPLC-HRMS. The method demonstrates low detection limits (as low as 0.05 ng/mL) and good reproducibility (RSD < 15%), and its performance was validated through blind samples, administration studies, and comparison with traditional IRMS. This approach provides a practical solution for rapid and accurate screening and confirmation of steroid esters, enhancing anti-doping capabilities.