Development of a hepatic cryoinjury model to study liver regeneration

We developed a new model to study liver regeneration based on cryoinjury. Hepatic cryoinjury induced a localised necrotic and apoptotic lesion characterised by inflammation and infiltration of innate immune cells. Following this initial phase, we observed fibrosis, which resolved as regeneration re-established homeostasis in 30 days. Importantly, this approach enables the comparison of healthy and injured parenchyma with an individual animal, providing unique advantages to previous models. These datasets include Bulk RNA-seq of livers collected at sham, 1 dpci, 3 dpci, and 7 dpci. Adult zebrafish were cryoinjured, collection of samples for Bulk RNA-seq was done at different time points, including sham, 1, 3 and 7 days-post cryoinjury (dpci)