Expression data from Arabidopsis thaliana depleted of HYPK or NAA10

N-alpha terminal acetylation is an abundant protein modification in eukaryotes. The NatA (N-alpha acetyltransferase A) complex is responsible for N-terminal acetylation of majority of the cytosolic proteins and its core is composed of NAA10 and NAA15 subunit. HYPK has been shown to interact with NatA core complex in human and fungus, modulating its activity. Here we address the in vivo function of the plant HYPK protein. A transcriptomics approach was applied to compare transcriptional reprogramming induced by depletion of NAA10 or HYPK mutants in plants. 6-week-old plants grown on soil under short-day conditions were used for RNA extraction and hybridization of RNA to Affymetrix arrays. 4 samples of each WT (control), NAA10 knock-down line (amiNaa10) and HYPK knockout lines (hypk-1, hypk-3) were chosen.