Data_Sheet_1_Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells.PDF

In general, neurodegenerative disorders have a great deal of correlation with the misfolded as well as aggregated forms of protein-based macromolecules. Among various species formed during the aggregation process, protein oligomers have been classified as most toxic entities against several types of living cells. A series of chemicals have been developed to inhibit protein aggregation as a measure to regulate neurodegenerative diseases. Recently, various classes of nanoparticles have also been reported to inhibit protein aggregation. In the present study, we synthesized fluorescent gold nanoparticles (B-AuNPs) employing Olax scandens leaf extract. Next, an in vitro study was performed to assess the effect of as-synthesized B-AuNPs on the aggregation behavior of the ovalbumin (OVA) and other related model proteins. We performed an extensive study to elucidate anti-amyloidogenic properties of nano-sized entities and established that small-sized B-AuNPs manifest chaperone potential against protein aggregation. Further, we exploited as-synthesized B-AuNPs as a mean to prevent protein aggregation mediated toxicity in neuroblastoma cells.

总体而言，神经退行性疾病与基于蛋白质的大分子（如错误折叠和聚集形式）之间存在着密切的相关性。在聚集过程中形成的各种物种中，蛋白质寡聚体被归类为对多种细胞类型最具毒性的实体。一系列化学物质已被开发出来以抑制蛋白质聚集，作为调节神经退行性疾病的一种手段。最近，也有报道指出各种类别的纳米颗粒也能抑制蛋白质聚集。在本研究中，我们利用南蛇藤叶提取物合成了荧光金纳米颗粒（B-AuNPs）。随后，进行了一项体外研究，以评估所合成的B-AuNPs对卵清蛋白（OVA）及其他相关模型蛋白聚集行为的影响。我们进行了一系列研究，旨在阐明纳米尺度实体的抗淀粉样蛋白生成特性，并证实了小尺寸的B-AuNPs展现出对抗蛋白质聚集的伴侣潜能。此外，我们还利用所合成的B-AuNPs作为一种预防神经母细胞瘤细胞中蛋白质聚集介导的毒性的手段。