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qRT-PCR analysis of relative transcript abundance compared to mRNA-Seq data*.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_qRT_PCR_analysis_of_relative_transcript_abundance_compared_to_mRNA_Seq_data_/250192
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*Selected cDNAs with abundant (APN3, APN4) and few (FT1, FT2) transcripts in the soybean aphid gut transcriptome were amplified by qRT-PCR with GAPDH as internal control. The relative abundance of each transcript as determined by qRT-PCR is shown alongside the numbers of short read sequences assembled for each cDNA.adepth (coverage per nt) = number of reads x 75 nt/contig length.
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2015-12-02
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