Bulk RNAseq of A375 cells treated with Panobinostat

The MICA and MICB genes are epigenetically regulated by HDACs. We tested if panobinostat, a broad spectrum HDAC inhibitor, affects the expression of MICA and MICB gene by a human melanoma cell line. Parental A375 cells were treated for 24 hours with panobinostat (50 nM) or the corresponding volume of PBS. Cells were then detached with Versene, and RNA was isolated with RNeasy Plus Mini Kit and RNase-Free DNase Set, respectively (both Qiagen kits, catalog numbers 74134 and 79254, respectively). Generation of cDNA, sequencing, and analyses were done as previously reported (Ferrari de Andrad et al, Science 2018, 359(6383)1537).