Recovery of hemodynamic parameters of hearts subjected to perfusion, IPC+‘delay’+I/R, and I/R with or without protease inhibitors.

Delay- hemodynamic recovery of hearts undergoing I/R with or without IPC+‘delay’. Proteases inhibitors- hemodynamic recovery for IPC+‘delay’+I/R in groups of hearts receiving: an inhibitor cocktail (leupeptin 11.7 µmol/L; pepstatin A 4.4 µmol/L; MG132-3 µmol/L), a proteasomal inhibitor (MG132-3 µmol/L), a lysosomal protease inhibitors cocktail (leupeptin 11.7 µmol/L, pepstatin-A 4.4 µmol/L). The inhibitors were applied subsequent to the IPC procedure followed by the ‘delay’ period and then subjected to I/R. For experimental protocols see figure 1 groups 4–6 and 10. Controls (considered 100%) are the heart’s hemodynamic parameters at the end of a 10 min perfusion period (end of the stabilization period). Results represent means±SE. The numbers in parentheses indicate the number of repetitious experiments. *Significantly different from controls-perfusion only (p<0.05). † = significantly different from IPC+I/R (p<0.05).