MHC Genometyping of Indian Rhesus Macaques

Indian rhesus macaque major histocompatibility complex (MHC) variation can influence the outcomes of transplantation and infectious disease studies. Frequently, rhesus macaques are MHC genotyped to identify variants that could account for unexpected results. Since the MHC is only one locus in the genome where variation could impact experimental outcomes, methods for simultaneously profiling variation in the macaque MHC and the remainder of the protein coding genome would be useful. Here we introduce macaque MHC genometyping, in which MHC class I and class II genotypes are determined with high confidence as part of a workflow that is commonly used for conventional exome analysis.