Gene expression of subpopulations arising from a nitrogen down-shift with proline as a nitrogen source in S. cerevisiae

When S. cerevisiae cells growing exponentially in rich media (YPD) are shifted to nitrogen-limited media with proline (0.8 mM) as a nitrogen source, they differentiate into two subpopulations of different phenotypes including cell size, growth and survival capabilities. The RNA-seq data presented here are for each subpopulation (5 biological replicates per subpopulation) sampled 1 hour after the shift and separated by FACS. Overall design: We compare 5 biological replicates for each subpopulation. Strain is a prototrophic version of BY4741 containing a subpopulation marker (pRPL28) identified by scRNAseq.