Selection criteria for designing comparative microsatellite primers.
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Lexpected: expected length of PCR products (bp); Lprimer: primer length (bp); Tm: melting temperature (°C); ΔTm: Tm difference between both primers; %GC: G+C content; 2-6×: number of tandemly repeated non-mononucleotide motifs (2–6 bp); 1×: length of mononucleotide runs; ΔG: Gibbs free energy required to break the secondary structure (kcal/mol); 3′: 3′-end of primers; Int: Internal; HP: hairpin, SD: self-dimer, CD: cross-dimer.*Output from NetPrimer; criteria as recommended in the application's manual.†Exceptionally up to 26 bp.‡Output from PrimaClade.§A 30–62% range was tolerated for primers >22 bp.
创建时间：
2015-12-02
