Amines and lipids metabolites in blood plasma and saliva samples in pigs.

The dataset presented in here is generated in a project named "Effects of sanitary and health status on amino acid and energy metabolism of growing-finishing pigs." The metabolomics data from two samples types in pigs were generated in collaboration with Metabolomics Facility Leiden, The Netherlands and Wageningen Livestock Research, The Netherlands. This collaboration was realized and funded by Enabling Technology Hotels programme, ZonMW, NWO, The Netherlands (project number: 435005015). 

Targeted quantification of metabolites in two metabolomic platforms covering  amines and oxidative stress metabolites in the blood and saliva samples in pigs. The samples were collected from a feeding trial. Briefly, After weaning, i.e., at week 4, pigs were fed a starter (4-9 weeks), grower (9-14 weeks), and finisher (14-22 weeks) diet containing either starch or fat as an energy source. At week 9, before the pigs were fed the grower diet, blood plasma and saliva samples were collected from the pigs (n=6) and the animals were stratified according to different hygiene conditions. At week 14, i.e., before the pigs received the finisher diet, and at week 22, i.e., at the end of this experiment, blood plasma and saliva samples were collected from the pigs (n=6) in the cohort receiving a diet with a different energy source under contrasting sanitary status. 

Targeted quantification of metabolites in two metabolomic platforms covering  amines and oxidative stress metabolites in the blood and saliva samples in pigs. The number of identified metabolites are shown in Table 1.

Table 1: Number of identified amines and lipids metabolites in blood plasma and saliva samples in pigs.  

Data reported as

Peak areas1

Relative response ratios4

Confidence2

Caution3

Confidence

Caution

Amines

Blood plasma

not required

not required

58

2

Saliva

not required

not required

52

5

Lipids

(low pH)

Blood plasma

47

17

47

17

Saliva

18

34

52

11

Lipids

(High pH)

Blood plasma

24

25

24

25

Saliva

28

17

28

17

1 For the lipid platform, large variations in internal standard were observed between study samples. This could be due to the difference in matrix effect between the study samples, i.e., blood plasma and saliva. It is known that the matrix effect varies significantly depending on the origin of the samples and is influenced by phenotypic characteristics such as species, age, and gender. Therefore, peak areas were provided as an additional data set that can be used as input data for downstream metabolomics analysis.

2 Metabolite signaling complied with the acceptance criteria of RSDqc <15%.

3 Metabolite signaling did not comply with the acceptance criteria of our quality control i.e. of RSDqc <15%, but they present RSDs up to 30%.

4 target area/ISTD area; unit free.

Available data-set:

-Four different signaling lipids data-set: 1) peak areas for plasma samples, 2) peak area ratios (metabolite to ISTD) for plasma samples, 3) peak areas for saliva samples, and 4) peak area ratios (metabolite to ISTD) for saliva samples.

 - Two signalling amine data-set: 1) peak area ratios (metabolite to ISTD) for plasma samples, and 2) peak area ratios (metabolite to ISTD) for saliva samples.