Profiling the functionality of designed transcription activation domain sequences to identify their grammar rules and exceptions

To investigate the functionality of sequences as transcription activation domains (TADs), a library was screened for growth on aureobasidin-containing growth media. Libraries of designed sequences were investigated to identify certain features associated with TAD functionality, such as balance between acidic and aromatic amino acids, and preferential location of certain types of amino acids. Overall design: DNA-Seq of yeast cell line Y2H Gold containing designed TAD sequences, at baseline and after growth on growth medium containing antibiotic aureobasidin for up to four days. Five biological replicates were taken at each time point, indicated by biorep barcodes appended to the sequences. Ability to grow on antibiotic-containing media leads to increase in sequence read counts after a certain growth period, demonstrating the functionality of certain TAD sequences