Identification of yellow seed color genes by BSR-Seq in Brassica juncea L.

Yellow seed breeding is an effective method to improve oil yield and quality in rapeseed (Brassica napus), but no natural occurring yellow-seeded genotypes was found in B. napus. Mustard (Brassica juncea) has some natural yellow-seeded germplasms, but the molecular mechanism of B. juncea is not clear. A BC9 population derived from the cross of yellow seed mustard Wuqi and brown seed mustard Wugong was used to analyze the candidate genes controlling yellow seed color in B. juncea. Then yellow-seed (BY) and brown-seed (BY) bulks were constructed in the BC9 population and were used for bulked segregant RNA-Sequence (BSR-Seq).