Proposed cut-off values for the in silico tools that discriminate AG-dependent 3′ss from AG-independent 3′ss.

aThe cut-off values were proposed according to the predicted values obtained using the test set of naturally occurring G+1 mutations (as used in Tables 1 and 2). bThe sensitivity and specificity calculated using Fu-mut set of G+1 mutations, containing artificially mutated PPTs. cThe sensitivity and specificity calculated using the original test set of G+1 mutations. The predicted scores and percentiles below the cut-off values and the differences between the predicted values for wild type and mutant sequences above the cut-off values are supposed to pertain to variants prone to affect splicing.