Homo sapiens Genome sequencing and assembly. Homo sapiens

The aim of this study was to dissect different type of extracellular RNA released by a human mast cell line (HMC-1). Extracellular RNA was isolated from the cell culture media and separated into two entities on a density gradient, a high density and a low density fraction. Both a long and a short RNA library was constructed and sequenced from the high and low density samples as well as the cells. Additional, miRNA and mRNA was analysed with microarray and the protein content was determined with mass spectrometry. These results show that cells can release multiple types of extracellular RNA with substantial differences in RNA species content. This is important for any future studies determining the nature and function of extracellular RNA released from different cells under different conditions.