Effect of High Fat Diet on gene expression of liver from obesity-prone (C57BL/6J) and obesity-resistant (A/J) mice. Mus musculus

Mice were fed for 2, 10 or 30 days with a normal chow (NC) or a high fat diet (HFD). At each time point, mice were sacrificed and liver samples were collected and immediately frozen in liquid nitrogen. Total RNA from each sample was further extracted, purified, quality-controled before and after amplification. Cy3-labeled RNA from individual liver sample was co-hybridized with a Cy5-labeled pool of RNA on the mouse 17K microarray. Keywords: diet response, time course, mouse strains Overall design: For a given time point, each individual RNA sample was hybridized with a pool of RNA from 6 mice