MAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT

In this study we take an integrative approach to capturing targets of the Hfq- associated sRNA PinT, a known post-transcriptional timer of the two major virulence programs of Salmonella enterica, using MS2 affinity purification and RNA- sequencing (MAPS). MS2 affinity purification and RNA- sequencing (MAPS) to identify PinT ligands in bacteria under in-vitro conditions mimicking specific stages of the infection cycle, and in bacteria growing inside macrophages.