Antibody responses of mice to flea salivary gland extract after exposure to uninfected flea bites.

aELISA results; number positive out of total (No. +) and the mean units of antibody (log10 (U) ± s.d.) of the positive samples are shown. Positive samples defined as having a mean log10 (U)>2 s.d. above negative control sera.bFleas fed on the abdomen of the mice. At the end of their 10-week exposure period, Groups A, B and C mice used to evaluate sensitization also received a final exposure of 25 fleas on the ear 12 hours prior to euthanasia.cmean total cumulative bites received per mouse.All 5-week samples were negative for IgM and all samples were negative for IgE.Antibody responses of mice to flea salivary gland extract after exposure to uninfected flea bites.