Triggering of Endoplasmic Reticulum Stress via ATF4-SPHK1 Signaling Promotes Glioblastoma Invasion and Chemoresistance

After treated with temozolomide for 3 days, the total RNA of LN229 cells was obtained and reverse transcribed into cDNA. The conduction of sequencing was on Illumina HiSeq platform by Personal Biotechnology company (Shanghai, China). Aligning reads of each sample to the human genome (Homo sapiens. GRCh38) was performed. Differential expression genes were characterized by using the DESeq2 with expression difference fold |log2FoldChange| > 1 and pvalue < 0.05.