Abiotic stressors such as extreme temperatures and pesticide exposure can impair stored sperm viability within queen honey bees, but little is known about how these stressors may directly impact queen performance or other quality metrics. Here, in a blind field trial, we exposed queens to cold temperatures (4 C, 2 h), hot temperatures (42 C, 2 h), a pesticide cocktail of xenobiotic compounds commonly found in wax, a solvent control, and a handling control. We used proteomics to investigate potential vertical effects of heat stress on embryos, as well as to measure the abundance of previously determined protein markers for abiotic stressors in the spermathecal fluid.