C/EBPß regulates the M2 transcriptome in ß-adrenergic-stimulated macrophages

We screened RNAseq results from RAW 264.7 macrophages for previous empirically-defined M2 genes in the whole transcriptome that followed the Arg1 pattern of up-regulation by ß-adrenergic-signaling with inhibition by Cebpb gene knockdown. We also screened RAW 264.7 macrophages for empirically-defined M1 genes that exhibited the orthogonal pattern of Arg1 regulation (i.e., down-regulation by ß-adrenergic signaling, with rescue of this effect by Cebpb knockdown). Overall design: Total RNA was purified from RAW 264.7 macrophage cells that were incubated in one of four experimental conditions: Control siRNA, Cebpb siRNA, Control siRNA+Isoproterenol, Cebpb siRNA+Isoproterenol; four biological replicates per condition