Amblema plicata microsatellite data from wild and hatchery produced populations

The goal of this project was to compare the genetic diversity of a wild source population of Three-ridge mussels (Amblema plicata) to the cohort of juveniles propagated from three gravid females from the wild population. The juveniles were subsampled directly after transformation from glochidia (“young juveniles”) and subsampled again after one year of being raised in the hatchery (“old juveniles”).  After correcting for different sample sizes, the young juveniles exhibited the greatest allelic richness, followed by the source mussels and then the old juveniles. All three groups exhibited private alleles. Private alleles in the juveniles indicated the dams (mothers) were likely fertilized by males living upstream of the source population, outside of the sampling effort of this study. High levels of multiple paternity were observed in the young and old juveniles. In total, 89 juveniles were estimated to have been sired by 58 males, increasing the amount of genetic variability in the..., We collected non-destructive samples from 50 Three-ridge mussels in the Upper Cedar River near Lansing, Minnesota using buccal swabs, which were then stored in ethanol (the ‘source’ sample set). Three gravid females from the source population were used for propagation at the Center for Aquatic Mollusk Programs in Lake City, MN. Hatchery staff infested individual walleye (Sander vitreus) host fish with the glochidia from a single dam. After transformation and dropping from their hosts, 20 juveniles from each dam were collected and preserved in ethanol for a total of 60 samples (‘young juveniles’). Staff combined the remaining juveniles and reared them at the hatchery for one year. On July 22, 2020, staff collected 50 more juveniles and preserved them in ethanol (‘old juveniles’).
Genomic DNA was isolated from the buccal swabs of the adult mussels using QIAGEN’s Purgene Buccal Cell Core Kit A following the ‘DNA Purification from a Buccal Brush’ protocol (Qiagen). Genomic DNA was isolated ..., , # Amblema plicata microsatellite data from wild and hatchery produced populations

[https://doi.org/10.5061/dryad.08kprr5cp](https://doi.org/10.5061/dryad.08kprr5cp)

## Description of the data and file structure

The data was collected to do a genetic comparison of a wild source population of Three-ridge mussels ('source') to the juvenile population propagated from the source. The propagated juveniles were subsampled at two points in time, once directly after transformation from glochidia ('J1'), and again after being reared for a year in the hatchery ('J2').

### Files and variables

#### File: Ampl\_3pops\_2019.csv

**Description:**  This data is formatted for GenAlEx. 0s indicate no data-- either the locus did not amplify or the data was removed later due to null alleles. 

##### Variables

* 10: The number of loci.
* 134: Total number of samples. 
* 3: Number of 'populations'-- source (wild mussels), J1 (juveniles directly after transformation from glochidia), J2 (juveniles after a...,