Barley Genotypes for Vernalization genes

Identification of presence of the VERNALIZATION1 (VRN1) novel alleles in varieties and landraces of Wheat seed from the Australian Winter Cereal Collection (AWCC). The data was collected 2005-06. See Hemming MN, Fieg S, Peacock WJ, Dennis ES and Trevaskis B. 2009. Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Molecular Genetics and Genomics. 282: 107-117. for the key to the results.\n\nThis will allow different flowering behaviours to be identified amongst different Barley varieties and landraces held at the AWCC. For some lines, the data is preliminary as the seeds are not homogenous.\nLineage: Please see the included DNA extraction protocol.\n\nSee Hemming MN, Fieg S, Peacock WJ, Dennis ES and Trevaskis B. 2009. Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Molecular Genetics and Genomics. 282: 107-117. for other method details and key to results.\n\nPlease note that the data is preliminary, as some seeds are not homogenous. “Landraces” were frequently found to have mixed genotypes for VRN1 . The same is true for some modern varieties. Additionally, technical and operator error will reduce the accuracy of our genotyping data. Finally, other genes (eg. VRN3 ), which have not been examined, might also influence flowering behaviour. Despite these limitations, the genotype data does allow selection for particular flowering behaviour. For example, a large number of potential winter wheats can now be selected from amongst the AWCC collection. Similarly, large numbers of facultative barleys (early flowering in long days) could be identified using these genotype data. We would recommend subsequent flowering time tests or re-genotyping (multiple seeds per line, or single seed descent) to confirm our initial genotype scores.