Combination of high-dimensional approaches for skeletal muscle tissue cartography. Combination of high-dimensional approaches for skeletal muscle tissue cartography

The regenerative capacity of skeletal muscle relies on muscle stem cells (MuSCs, or satellite cells) and its niche interactions with different neighboring cells. To understand the cellular diversity within adult skeletal muscle tissue, we harvested mononuclear cells from hindlimb skeletal muscles, sorted into single cells and profiled them by single-cell RNA-seq. To further understand and compare the expression profile between MuSCs and a novel smooth-muscle/mesenchymal-like cells (SMMCs) population, we isolated the two cell types by FACS and profiled them respectively by bulk RNA-seq. Overall design: (scRNA-seq) Mononuclear cells from hindlimb skeletal muscle from wild type mouse were profiled by taking advantage of 10X Chromium platform. (bulk RNA-seq) MuSCs and SMMCs cell population were isolated from hindlimb skeletal muscle from wild type mouse. Their polyA+ RNA were profiled using Smart-seq2.