Global hepatic transcript data from fasted male BXD strains on chow or high fat diet

Transcript data from livers from fasted-state BXD strains on chow or high fat diet We used microarrays to compare the hepatic expression differences across the BXD strain family and across two diverse diets 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused, then livers were snap-frozen in liquid nitrogen for RNA extraction and RNEasy cleanup. Each dietary and strain cohort consisted of ~5 animals which were prepared independently then pooled evenly by µg RNA before the Affymetrix arrays were run.本数据集为禁食状态下喂食普通维持饲料或高脂饲料的BXD品系小鼠肝脏转录组数据。研究采用基因表达微阵列（microarrays），比较BXD品系小鼠家族在两种不同饲料喂养条件下的肝脏基因表达差异。实验对象为29周龄雄性小鼠，于晚间6时至次日上午9时禁食过夜，经异氟烷（isoflurane）麻醉后实施灌流，随后将肝脏迅速置于液氮中冷冻保存，用于RNA提取及RNeasy试剂盒纯化。每个饲料-品系组群包含约5只独立制备的小鼠，在开展Affymetrix芯片检测前，按每微克RNA的量将各组样本均匀混合。