HMG box mutation of TCF-4 in non-small cell lung cancer

T-cell factor 4 (TCF-4) is determined to play a crucial role in Wnt/β-catenin signaling pathway activation. The high mobility group (HMG) box domain of TCF-4 contributes to interacting with DNA motif for transcriptional regulation. Herein, we generated non-small cell lung cancer (NSCLC) cell line A549 with stably over-expressed TCF-4 with HMG box hot spot mutation (10th exon partial deletion), and conducted TCF-4 and β-catenin ChIP-Seq to explore the differential genomic binding patterns. Our data determined the important role of TCF-4 in gene transcription controlling and provided the gain function evidence of TCF-4 caused by the TCF-4 mutation in NSCLC.