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VTA and NAC labeled ribosome from mPFC

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101185
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Projection-dependent ribosome profling from mouse mPFC. Ribosome from NAC- and VTA- projecting mPFC cells were immunoprecipited using GFP-trap (Chromotech). Translating mRNA was isolated and analyzed Cre-dependent ribosome TRAP mice were injected with CAV2-Cre in NAC and VTA. Projection-dependent ribosome labeling was enabled by isolating mPFC. Ribosome was genetically tagged with GFP in the activated cells and could be immunologically purified.
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2021-07-25
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