Patterns of transcript expression and determination of reliably expressed transcripts.

A, Transcripts that occur at low abundances are more frequent than transcripts that occur at high abundances. Power-law distribution states that the probability of gene expression k, will decay as a power-law P(k) ∞ k−r. This expression pattern occurs in both neuronal types. DRG R2 = 0.927. CGN R2 = 0.902. Axis in log scale. B, Logistic regression relationships between ‘reliability’ of isoform expression, measured by isoform FPKM having a lower confidence limit exceeding 0, and log (FPKM), for DRG neurons (left) and CGNs (right).