The characterization of six GH5 genes expressed in E.coli.
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1Nearest neighbor represented nearest protein sequence for each GH5 genes.2All activity assays were performed at pH7.4 and 50°C. The substrates used to determine activities of endoglucanase, mannanase, xylanase and exocellulase were CMC, locust bean gum, xylan (beechwood) and pNPC. The unit of enzyme activity is U/mg protein.3Activity was not detected.4Activity was less than 1 U/mg protein.5Cel5 and Cel6 were also mentioned as man1 and en2 in elsewhere (Yan et al., 2013), but were not expressed before.The characterization of six GH5 genes expressed in E.coli.
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2015-12-03
