Deep Learning-based discovery of tetrahydrocarbazoles as broad-spectrum antitumor agent and its click-activated prodrug strategy

HCT116, RKO, RBE and HUCCT1 were cultured in 6-well plates (triplicates), which were treated with vehicle or WJ0909b at concentration of 200, 100, 60, and 160nM for 48 hours. Cells were washed with PBS twice, and then lysed with TRIzol reagent (Invitrogen, 15596018).