Rate of formation of ePL kinase•PLP complex in the presence of different substrates and productsa.
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aePL kinase (90 µM) samples were incubated with different combinations of PL (150 µM), PLP (150 µM), MgATP (400 µM), MgADP (400 µM) and MgCl2 (0.2 mM). Complex formation was measured at time intervals as described in Materials and Methods section. Values are the average of three independent measurements and the standard deviation was less than 10%.
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2015-12-02
