The parallel reaction monitoring (PRM) target sequencing based on the comparative proteomic profiling in sheep spermatozoa under non-capacitating (NC) and capacitating (CAP) conditions in vitro using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with tandem mass tag (TMT) labeling strategy

To verify protein expression levels obtained by TMT LC-MS/MS analysis strategy, the expression levels of differentially abundant proteins in NC and CAP groups were selected and quantified by a PRM assay.