SMAD7 binds to SMURF2

SMURF2, an E3 ubiquitin protein ligase, binds to SMAD7 in the nucleus. WW2 and WW3 domains of SMURF2 are both required for binding PY motif (PPXY sequence) of SMAD7. Endogenous human SMAD7 and SMURF2 were shown to form a complex in human U4A/Jak1 cells, derived from a sarcoma cell line 2fTGH. The interaction was studied in more detail by expressing tagged recombinant human SMURF2 and mouse Smad7 in human embryonic kidney cell line HEK293 (Kavsak et al. 2000, Ogunjimi et al. 2005).

SMURF2（一种E3泛素连接酶）在细胞核中与SMAD7结合。SMURF2的WW2和WW3结构域对于与SMAD7的PY基序（PPXY序列）的结合至关重要。研究表明，内源性人类SMAD7与SMURF2在源自肉瘤细胞系2fTGH的U4A/Jak1细胞中形成复合物。通过在人类胚胎肾细胞系HEK293（Kavsak等，2000年，Ogunjimi等，2005年）中表达标记的重组人SMURF2和小鼠Smad7，进一步详细研究了这一相互作用。