Transcriptome analysis of medaka (Oryzias latipes) exposed to tributyltin. Oryzias latipes

Tributyltin is an organotin compound that disrupts the endocrine system of aquatic organisms, and its obesogenic toxicity to various species is well known. However, the mechanism of TBT to disrupt the endocrine system is not well known. Therefore, to investigate the effects caused by TBT in fish, we exposed tributyltin to juvenile medaka (Oryzias latipes) and analyzed the gene expression changes using mRNA-Seq. As a result of mRNA-Seq analysis, it was cleared that toxicity related genes, such as cytochrome P450 superfamily genes connected to hormonal metabolism, and proliferator-activated receptor signaling pathway genes related with obesity, were significantly affected by TBT. Our results of mRNA-seq analysis simultaneously demonstrated the mechanisms of TBT toxicity in TBT exposed medaka. mRNA-Seq could be a strong tool to investigate and clear the toxic effects caused by pollutants.