Effect of Tat-SF1 depletion on basal and Tat-dependent HIV-1 transcription in HeLa cells*.
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*Values shown are the means of duplicate wells for 3 independent experiments. Row 1 is calculated from the same experiment shown in Figure 4B. CAT activity was the value of the slope of the linear function obtained by plotting cpm of acetylated chloramphenicol versus time. Luciferase activity of the same sample was measured with a luminometer. For both reporter assays, background values obtained from lysis buffer alone were subtracted from each sample.
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2015-12-02
