Dataset on particle size of Bacillus subtilis and Chromobacterium violaceum cultivated in different growth medium

Particle size is an important parameter for assessing the “health status” of microbial cells. For example, cells deficient in the cell division machinery may be concatenated into long filaments. This dataset describes some initial observations of number-average particle size of <i>Bacillus subtilis</i> NRS-762 (ATCC 8473) and genetically engineered and wildtype <i>Chromobacterium violaceum</i> ATCC 12472 cultivated in different growth media over different cultivation periods. At appropriate timepoint, samples were pipetted from the culture broth into 15 mL polypropylene centrifuge tube. Dilution was applied when necessary to profile for any dilution related effect on particle size distribution. Vigorous shaking by hand was used to disperse any cell clusters prior to particle size analysis, which was carried out with the pipetting of sample into a square cuvette for analysis in Malvern Zetasizer Nano ZS instrument.