Guide RNA off-target predictions in the <i>L</i>. <i>donovani</i> luciferase expressing genome using Cas-OT.

Columns list the number of sequences that match the guide RNA sequence with a number of mismatches in the seed region and non-seed region of the protospacer adjacent motifs. A00 is a perfect match, A10 is the number of sequences in the genome that match with a single mutation in the seed region. A05 lists the number of sequences that match the guide sequence with 5 mutations in the more permissive non-seed region. All guides should have a single match for A00, the targeted cut site where the genome has been assembled. Guides highlighted in red do not have a perfect match to the assembled genome strain, but do perfectly match the UTR’s identified from the unassembled genome of the experimental strain. (XLSX)