SDS-PAGE and protein content quantification assay of zein, non-zein and total protein extracted from mature kernels.

(A–C) SDS-PAGE assay of zein (A), non-zein (B) and total protein (C) contents in WT, FA7, FA9 and FA12 mature kernels. (D–E) Quantification of zein (D), non-zein (E) and total protein (F) contents in WT, FA7, FA9 and FA12 mature kernels. Significant differences between the transgenic lines and WT were evaluated by Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).